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CD4, PE-Cyanine7, clone: YKIX302.9, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-5040-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15548556

  • £171.42 / 100 tests


EU Stock 14
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Description

Description

Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells. In dogs, CD4 is also highly expressed on neutrophils. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis.This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and in vitro.Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YKIX302.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P33705
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Flow Cytometry
YKIX302.9
PE-Cyanine7
CD4
Conjugated
CD4
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
Canine
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