CD4 Mouse anti-Human, Brilliant Violet 750, Clone: SK3 (also known as Leu3a), BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15834979	BD Biosciences 566356	25 μg
15824979	BD Biosciences 566355	50 μg

Description

The SK3 monoclonal antibody specifically binds to CD4. The CD4 antigen is a 55 kDa type 1 transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed on T-helper/inducer lymphocytes and monocytes. CD3+CD4+ T cells comprise approximately 28% to 58% of normal peripheral blood lymphocytes. CD4 is also expressed on 80% to 95% of normal thymocytes. The CD4 antigen is present in low density on the cell surface of monocytes and in the cytoplasm of monocytes and macrophages (CD3-CD4+). CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. BV750

Specifications

• Antigen: CD4
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 750
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human Peripheral Blood T Cells
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: SK3 (also known as Leu3a)
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Gene Alias: L3T4, Leu3a, T-cell surface antigen T4/Leu-3, W3/25, CD4 antigen (p55)
• Isotype: IgG1 κ
• Purification Method: Affinity Purified