CD4 Mouse anti-Human, Brilliant Violet 421, Clone: RPA-T4, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15831909	BD Biosciences 562425	25 Tests
15821909	BD Biosciences 562424	100 Tests

Description

The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations. CD4 is also expressed on thymocytes subsets and at lower levels on monocytes and macrophages. CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV). This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Specifications

• Antigen: CD4
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 421
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: RPA-T4
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: L3T4; T-cell surface antigen T4/Leu-3; W3/25; CD4 antigen (p55)

For Research Use Only.