CD4 Monoclonal Antibody (RPA-T4), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 16-0049-025

Product Code. 17130902

Description

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays. Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD4 Monoclonal antibody specifically detects CD4 in Human samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P01730
• Host Species: Mouse
• Quantity: 25 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: RPA-T4
• Conjugate: Functional Grade
• Gene: CD4
• Gene Alias: CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 receptor; CD4, allele 1; cd4a; CD4mut; cell surface glycoprotein CD4; fCD4; L3T4; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); W3/25; W3/25 antigen
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 920
• Content And Storage: 4° C
• Form: Liquid