CD4 Monoclonal Antibody (4SM95), eFluor™ 570, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-9766-80

Product Code. 15548966

Description

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Description: This 4SM95 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.The 4SM95 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD4 using immunohistochemistry of frozen tissue please refer to alternate products Anti-Mouse CD4 Purified (cat. 14-0041 and 14-0042).Applications Reported: This 4SM95 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.Applications Tested: This 4SM95 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P06332
• Gene Symbols: Cd4
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12504
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 4SM95
• Conjugate: eFluor 570
• Gene: Cd4
• Gene Alias: CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.