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IBA Lifesciences CD4 Fab-TACS™ Gravity Kit, Mouse

Product Code. 16232931 Shop All IBA Lifesciences Products
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Quantity:
10 Columns
4 Columns
Unit Size:
10 sets
4 sets
This item is not returnable. View return policy

Product Code. 16232931

Brand: IBA Lifesciences 63401010

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For manual positive cell separation. The Fab-TACS™ Gravity Kit provides label-free target cells without the use of high affinity antibodies and magnetic beads

  • The kit allows the selection of CD4+ T cells from mouse spleen.
  • Fab-TACS™ Gravity column (Capacity: 1x108target cells each)
  • CD4 Fab-Strep, mouse
  • Buffer CI (10x)
  • Biotin stock solution
  • Fab-TACS™ Gravity Adapter
  • The Fab-TACS™ Gravity column is filled with a Strep-Tactin™ coated matrix made of cell-grade agarose.
  • Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix.
  • Subsequently, single-cell tissue suspensions pass through the column.
  • Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell.
  • Non-target cells are efficiently washed away.
  • In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
  • After elution, the Fab-Streps self-dissociate from the cell surfaces.
  • The label-free authentic target cells are now ready for further downstream applications.
  • Specifications

    Content And Storage 2°C to 8°C
    Quantity 10 Columns
    Type Non-magnetic Cell Isolation Kit
    Species Mouse
    Sufficient For 1x108 target cells/column
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