CD3e Hamster anti-Mouse, PerCP-Cy5.5, Clone: 145-2C11, BD

Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15850779	BD Biosciences 561108	25 μg
15896558	BD Biosciences 551163	0.1 mg

Description

The 145-2C11 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex that is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events that activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes. Preincubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining reportedly enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes.

Specifications

• Antigen: CD3e
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Hamster
• Target Species: Mouse
• Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: 145-2C11
• Applications: Flow Cytometry
• Conjugate: PerCP-Cyanine5.5
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: CD3; CD3 epsilon; Cd3e; CD3ε; T3e

For Research Use Only.