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CD3e, PE, clone: 145-2C11, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0031-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15268419

  • POA / 500µg

This item cannot be ordered online because the price is subject to change. Please contact our Product Support team on 01509 555500 for assistance.

Disclaimers:

For Research Use Only.


Description

Description

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3e
145-2C11
PE
Conjugated
Armenian Hamster
500μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD3 epsilon
IgG
RUO
Primary
Murine
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