Invitrogen™ CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PE, eBioscience™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15527036	Invitrogen™ 12-5859-80	25 μg
15537036	Invitrogen™ 12-5859-82	100 μg

Description

Description: The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family. The ligand is beta1, 3-glycan. Beta glycans are a main component in polysaccharides and specifically beta1,3-linked carbohydrates are found in the cell wall of fungi, plants and some bacteria. CD369 has been found to bind Candida, Aspergillis, Pneumocystis, Coccidioides and Mycobacterium. Calcium dependent binding to ligand signals through syk via the ITAM domain in the cytoplasmic portion and results in endocyotosis/phagocytosis and production of cytokines (IL-2, IL-6, IL-10, TNF alpha, IL-1 beta, IL-23). A direct role in Th17 and Th1 differentiation has been attributed to CD369 signaling. CD369 is expressed predominantly on myeloid cells (neutrophils, monocytes, macrophages, and dendritic cells). Low levels have also been reported on lymphocytes and mucosal epithelium. Applications Reported: This bg1fpj antibody has been reported for use in flow cytometric analysis. Applications Tested: This bg1fpj antibody has been tested by flow cytometric staining of mouse lysed whole blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optima...

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

Specifications

• Antigen: CD369 (Clec7a, Dectin-1)
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q6QLQ4
• Gene ID (Entrez): 56644
• Immunogen: Baculovirus-expressed Fc-containing protein.
• Product Type: Antibody

• Clone: bg1fpj
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: Clec7a
• Gene Alias: Beta-glucan receptor; beta-GR; BGR; CANDF4; CD369; CLEC7A; Clec7b; CLECSF12; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; DECTIN1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; RGD1565140; SCARE2; UNQ539/PRO1082
• Gene Symbols: Clec7a
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography