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CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright™ 702, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£160.00 - £319.00
Specifications
Antigen | CD366 (TIM3) |
---|---|
Clone | F38-2E2 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15973754
|
Affymetrix eBioscience
67-3109-42 |
100 Tests |
£319.00
Pack of 100 |
Estimated Shipment: 20-05-2024 Log in to see stock. |
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15963754
|
Affymetrix eBioscience
67-3109-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.Specifications
CD366 (TIM3) | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
Q8TDQ0 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
F38-2E2 | |
Flow Cytometry | |
Super Bright 702 | |
Mouse | |
Human | |
84868 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |