CD35 Monoclonal Antibody (To5), Biotin, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA517713

Product Code. 15383991

Description

MA5-17713 targets CD35/CR1 in FACS, IHC (F), and WB applications and shows reactivity with Human samples. The MA5-17713 immunogen is mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.

CD35 (CR1, Complement Receptor 1) is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 protein mediates cellular binding to particles and immune complexes that have activated complement. The CD35 complex is four different allotypes (C,A,B,D) C is 160 kDa; A is 190 kDa; B is 220 kDa and D is 250 kDa. CD35 is involved in the processing of immune complexes, promoting of binding and phagocytosis of C3b complexes and inhibition of complement activation. CD35 is on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukemias, follicular dendritic reticulum cells and is negative on basophils. Decreases in expression of CD35 protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in the CD35 gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants encoding different isoforms of CD35 have been characterized. An additional secreted form of CD35 have also been described but has not been fully characterized.

Specifications

• Antigen: CD35
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 4-5mg/mL BSA and 0.02% sodium azide
• Gene Accession No.: P17927
• Gene Symbols: CR1
• Immunogen: Mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Clone: To5
• Conjugate: Biotin
• Gene: CR1
• Gene Alias: C3 binding protein; C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); Complement Component (3b/4b) receptor 1 including Knops blood group system; complement component 3b/4b receptor 1 (Knops blood group); complement receptor type 1; CR1; KN; Knops blood group antigen
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1378
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid