CD336 (NKp44) Monoclonal Antibody (44.189), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-3369-85

Product Code. 15577786

Description

CD336 is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.

Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.The 44.189 antibody has been reported to facilitate CD336 crosslinking.Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis and functional assays (receptor crosslinking).Applications Tested: This 44.189 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.

Specifications

• Antigen: CD336 (NKp44)
• Classification: Monoclonal
• Concentration: 1mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: O95944
• Gene Symbols: NCR2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9436
• Content And Storage: Store at 2-8°C.
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Clone: 44.189
• Conjugate: Functional Grade
• Gene: NCR2
• Gene Alias: CD336, LY95, NCR2, CD336, CD336 antigen, LY95lymphocyte antigen 95 homolog (activating NK-receptor, NK-p44), lymphocyte antigen 95 (activating NK-receptor, NK-p44), Lymphocyte antigen 95 homolog, natural cytotoxicity triggering receptor 2, Natural killer cell p44-related protein, NK cell activating receptor (NKp44), NK cell-activating receptor, NKp44, NKP44, NK-p44dJ149M18.1
• Host Species: Mouse
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.