CD33 Monoclonal Antibody (WM-53 (WM53)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0338-95

Product Code. 17120462

Description

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells. Applications Reported: The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunocytochemistry staining. Applications Tested: The WM-53 (WM53) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD33 Monoclonal antibody specifically detects CD33 in Human samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Specifications

• Antigen: CD33
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P20138
• Host Species: Mouse
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: WM-53 (WM53)
• Conjugate: Unconjugated
• Gene: CD33
• Gene Alias: Cd33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; SIGLEC-3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 945
• Content And Storage: 4° C
• Form: Liquid