CD326 (EpCAM), Biotin, clone: G8.8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5791-80

Product Code. 15219859

Description

Description: The G8.8 monoclonal antibody reacts with the 40kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD326 (EpCAM)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q99JW5
• Gene Alias: Epithelial Specific Antigen, ESA, KSA, M4S1, MK-1, DIAR5, EGP-2, EGP40, KS1/4, MIC18, TROP1, TACSTD1, EPCAM, Epithelial cell adhesion molecule
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: G8.8
• Conjugate: Biotin
• Gene: Epcam
• Format: Conjugated
• Gene Symbols: Epcam
• Isotype: IgG2a, kappa
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C. Do not freeze.
• Primary or Secondary: Primary
• Gene ID (Entrez): 17075

For Research Use Only.