CD32 Mouse, anti-Human, Unconjugated, Clone: FLI8.26, STEMCELL Technologies

Mouse Monoclonal Antibody

Brand:  Stemcell Technologies 60135

Product Code. 17129171

Description

• The FLI8.26 antibody reacts with human CD32 (FcγRII), an ∼40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation, and platelet aggregation
• The FLI8.26 antibody cross-reacts with monocytes, granulocytes, and platelets
• CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc
• Clone FLI8.26 inhibits binding of the FcγRIIa-specific clone IV.3 antibody in flow cytometry analyses
• CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes
• These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the FLI8.26 antibody may be used as a blocking antibody to reduce non-specific binding

Specifications

• Antigen: CD32
• Classification: Monoclonal
• Conjugate: Unconjugated
• Quantity: 100 μg
• Target Species: Baboon, Cynomolgus, Rhesus Monkey, Chimpanzee, Human
• Cell Type: B Cells, Granulocytes and Subsets, Monocytes

• Applications: Flow Cytometry
• Clone: FLI8.26
• Host Species: Mouse
• Primary or Secondary: Primary
• Isotype: IgG2b κ