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CD319 (CRACC), PerCP-eFluor™ 710, clone: 162, eBioscience™

Mouse Monoclonal Antibody

£99.00 - £210.00

Specifications

Antigen CD319 (CRACC)
Clone 162
Target Species Human
Conjugate PerCP-eFluor 710
Isotype IgG2b, kappa, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15589216 View Documents Affymetrix eBioscience
46-2229-41
25 Tests £99.00
25 tests View alternative products
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Estimated Shipment date
from Supplier 06-07-2020
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15599216 View Documents Affymetrix eBioscience
46-2229-42
100 Tests £210.00
100 tests View alternative products
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Description

Description

Description: The monoclonal antibody 162 recognizes CRACC (CD2 like receptor for activating cytotoxic cells) also known as CD319 and CS1. It is a member of the SLAM receptor family. CRACC is a 66kDa glycosylated transmembrane protein which contains a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif). It is this domain, found in other proteins such as CD244 and NTB-A, that interacts with SH2 containing proteins such as SAP. Interestingly the ITSM domain in CRACC is SAP independent. Instead EAT-2 acts as the signaling molecule for CRACC. This has profound impact for patients with X-linked lymphoproliferative disease (contains a SAP mutation). It should be noted that a splice variant has been reported which deletes the ITSM domain. Applications Reported: This 162 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 162 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD319 (CRACC)
Human
IgG2b, kappa, kappa
Monoclonal
Liquid
Antibody
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
162
PerCP-eFluor 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.