CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 17-3171-82

Product Code. 15588076

Description

Description: The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predomitly expressed by IPCs in the naive mouse which represent sa very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8. The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs. Applications Reported: This eBio129c (129c) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio129c (129c) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

CD317 (BST2, PDCA-1) Monoclonal antibody specifically detects CD317 (BST2, PDCA-1) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD317 (BST2, PDCA-1)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8R2Q8
• Gene Symbols: Bst2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 69550
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio129c (129c)
• Conjugate: APC
• Gene: Bst2
• Gene Alias: 2310015I10Rik; bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; Bst2; BST-2; C87040; CD317; Damp1; DAMP-1; DAMP-1 protein homolog; GREG; HM1.24 antigen; NPC-A-7; PDCA-1; protein DAMP-1; TETHERIN
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.