CD31 (PECAM-1) Monoclonal Antibody (390), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 16-0311-025

Product Code. 17180982

Description

The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction. Applications Reported: The 390 antibody has been reported for use in flow cytometric analysis. 390 has also been reported in in vitro functional studies. Applications Tested: The 390 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD31 (PECAM-1) Monoclonal antibody specifically detects CD31 (PECAM-1) in Mouse samples. It is validated for Flow Cytometry, Functional Assay

Specifications

• Antigen: CD31 (PECAM-1)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q08481
• Host Species: Rat
• Quantity: 25 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Functional Assay
• Clone: 390
• Conjugate: Functional Grade
• Gene: Pecam1
• Gene Alias: C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; Fc receptor-like 4-like; GPIIA'; I79_008304; LOC100357605; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18613
• Content And Storage: 4° C
• Form: Liquid