CD30 Monoclonal Antibody (mCD30.1), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
17160982	Affymetrix eBioscience 16-0301-025	25 mg
17170982	Affymetrix eBioscience 16-0301-050	50 mg

Description

The mCD30.1 monoclonal antibody reacts with mouse CD30, a 105-120 kDa member of the TNFR superfamily. CD30 is not detectable on splenocytes, but can be upregulated on T cells stimulated in vitro through TCR with a peak expression around day 4-5 post-activation. CD30 is expressed by both CD8^+ and CD4^+ activated cells. It is suggested that CD30 regulates cytokine secretion by T cells and has a role in T cell development. Applications Reported: The mCD30.1 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro functional studies. Applications Tested: The mCD30.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD30 Monoclonal antibody specifically detects CD30 in Mouse samples. It is validated for Flow Cytometry, Functional Assay

Specifications

• Antigen: CD30
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: Ber-H2; CD30; CD30 antigen; CD30 molecule; CD30L receptor; cytokine receptor CD30; D1S166E; Ki; Ki-1; Ki-1 antigen; lymphocyte activation antigen CD30; sCD30; soluble CD30; TNF receptor superfamily member 8; Tnfrsf8; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8; Tumor necrosis factor superfamily member 8; tumor necrosis factor superfamily, member CD30
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: mCD30.1
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: Q60846
• Gene ID (Entrez): 21941
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: TNFRSF8