CD3 epsilon Antibody (CRIS-7), Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18144519	Novus Biologicals NBP2-44878-0.02MG	0.02 mg
18011544	Novus Biologicals NBP2-44878-0.1MG	0.1 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD3 epsilon Monoclonal specifically detects CD3 epsilon in Human, Monkey samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: CD3 epsilon
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Lines, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 916
• Immunogen: Stimulated human leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 20 kDa

• Clone: CRIS-7
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Monkey
• Gene Alias: CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
• Gene Symbols: CD3E
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes the -chain of CD3 (Workshop V; Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

For Research Use Only