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CD29 (Integrin beta 1), clone: KMI6, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0292-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15296887

  • £67.59 / 100µg


Estimated Shipment
03-05-2018
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Description

Description

Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alpha beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin.Applications Reported: This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions.Applications Tested: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD29
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P09055
FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, ITGB1, ITBG1D
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Western Blot
KMI6
Unconjugated
Itgb1
Purified
Itgb1
IgG2a, kappa
100μg
Store at 2-8°C.
Primary
16412
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