CD289 (TLR9) Rat anti-Mouse, Biotin, Clone: M9.D6, eBioscience™

Rat Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-9093-80


UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg

Disclaimers: For Research Use Only.

Product Code. 15340100

Quantity Price
1 £95.00 / 25µg
Estimated Shipment date
from Supplier 28-10-2016
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Description and Specification


Antigen CD289 (TLR9)
Applications Flow Cytometry (Intracellular Staining)
Applications Western Blotting
Clone M9.D6
Concentration 0.5mg/mL
Conjugate Biotin
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias TLR-9, toll-like receptor 9
Host Species Rat
Isotype IgG2a
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predominantly expressed as an intracellular protein, TLR9 is a ∽115-120kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.