CD278 (ICOS) Mouse anti-Human, PE-eFluor 610, Clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 61-9948-42

100TEST Anti-Human CD278 (ICOS) PE-eFluor 610

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg

Disclaimers: For Research Use Only.

Product Code. 15530587

Quantity Price
1 £306.50 / 100 tests
Estimated Shipment date
from Supplier 26-10-2016
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Description and Specification


Antigen CD278 (ICOS)
Applications Flow Cytometry
Clone ISA-3
Concentration 5μL (0.25μg)/test
Conjugate PE-eFluor 610
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Isotype IgG1
Quantity 100 tests
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27kDa and 29kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.