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CD276 (B7-H3), PE, clone: M3.2D7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5973-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15279209

  • £68.27 / 25µg


Estimated Shipment date
from Supplier 04-04-2018
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Description

Description

Description: The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.Applications Reported: The M3.2D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M3.2D7 antibody is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of mouse B7-H3 transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of mouse B7-H3 transfected cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD276 (B7-H3)
M3.2D7
PE
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL; 5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B7RP-2, B7RP2, B7H3
IgG2a
RUO
Primary
Murine
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