CD276 (B7-H3) Monoclonal Antibody (MIH35), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5937-050

Product Code. 17191372

Description

This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2). Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis. Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD276 (B7-H3) Monoclonal antibody specifically detects CD276 (B7-H3) in Human, Mouse samples. It is validated for Flow Cytometry, Functional Assay

Specifications

• Antigen: CD276 (B7-H3)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q5ZPR3, Q8VE98
• Host Species: Rat
• Quantity: 50 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Functional Assay
• Clone: MIH35
• Conjugate: Functional Grade
• Gene: CD276
• Gene Alias: 4Ig-B7-H3; 6030411F23Rik; AU016588; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD276; CD276 antigen; Cd276 molecule; CD276 molecule tv1; Costimulatory molecule; PSEC0249; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276; UNQ309/PRO352
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 102657, 80381
• Content And Storage: 4° C
• Form: Liquid