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CD275 (B7-H2), Biotin, clone: HK5.3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5985-81

Code : Z1

Additional Details : Weight : 0.25000kg

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Product Code. 13485289

  • £68.96 / 50µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ∽40kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis. HK5.3 is not recommended for use in immunohistochemical staining.Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD275 (B7-H2)
HK5.3
Biotin
Conjugated
Rat
50μg
Store at 2-8°C. Do not freeze.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B7RP1, ICOSL, GL50
IgG2a
RUO
Primary
Murine
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