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CD273 (B7-DC), clone: 122, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9972-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15268297

  • £246.08 / 500µg


Estimated Shipment
01-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 122 monoclonal antibody reacts with mouse B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages in the mouse. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. 122 has been demonstrated to block binding of TY25 (Cat. No.14-5986), another mAb specific for mouse B7-DC.Applications Reported: 122 has been reported for use in flow cytometric analysis.Applications Tested: The 122 antibody has been tested by flow cytometric analysis of mouse dendritic cells or mouse B7-DC transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD273 (B7-DC)
122
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
PD-L2
IgG2a
RUO
Primary
Murine
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