CD272 (BTLA) Mouse anti-Mouse, Biotin, Clone: 8F4, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-5956-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg

Disclaimers: For Research Use Only.

Product Code. 15239949

Quantity Price
1 £ 66.65 / 25µg
Estimated Shipment date
from Supplier 02-05-2017
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Description and Specification


Format Conjugated
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze.
Regulatory Status RUO
Quantity 25μg
Gene Alias B and T lymphocyte attenuator
Concentration 0.5mg/mL
Host Species Mouse
Monoclonal or Polyclonal Monoclonal
Isotype IgG1
Antigen CD272 (BTLA)
Applications Flow Cytometry
Clone 8F4
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Conjugate Biotin

The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining. However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7.