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CD27, PerCP-eFluor™ 710, clone: LG.7F9, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0271-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341240

  • £70.65 / 25µg


EU Stock 3
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Description

Description

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD27
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P26842, P41272
CD27 antigen, CD27L receptor, T cell activation antigen CD27, T cell activation antigen S152, T-cell activation antigen CD27, tumor necrosis factor receptor superfamily member 7
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Murine, Rat
Flow Cytometry
LG.7F9
PerCP-eFluor 710
CD27
Liquid
CD27
IgG
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
21940, 500318, 939
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