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CD27, clone: LG.7F9, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0271-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15226877

  • £61.87 / 100µg


Estimated Shipment
31-07-2018
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Description

Description

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Use of mild detergent lysates is recommended when performing immunoprecipitation with this mAb. LG.7F9 has also been reported in blocking of ligand binding. (Please use Functional Grade purified LG.7F9, cat. 16-0271, in functional assays.).Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD27
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P26842, P41272
CD27 antigen, CD27L receptor, T cell activation antigen CD27, T cell activation antigen S152, T-cell activation antigen CD27, tumor necrosis factor receptor superfamily member 7
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurineþRat
Flow CytometryþFunctional AssayþImmunoprecipitation
LG.7F9
Unconjugated
CD27
Purified
CD27
IgG
100μg
Store at 2-8°C.
Primary
939
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