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CD27, Functional Grade, clone: LG.3A10, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0272-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278407

  • £62.25 / 50µg


EU Stock 5
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Description

Description

Description: The LG.3A10 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.8F10 cross-reacts with human and rat CD27.When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction.Applications Reported: This LG.3A10 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.Applications Tested: This LG.3A10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD27
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P26842, P41272
CD27 antigen, CD27L receptor, T cell activation antigen CD27, T cell activation antigen S152, T-cell activation antigen CD27, tumor necrosis factor receptor superfamily member 7
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)
LG.3A10
Functional Grade
CD27
Purified
CD27
IgG
50μg
Store at 2-8°C.
Primary
939
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