missing translation for 'onlineSavingsMsg'
Learn More

CD254 (RANK Ligand), clone: IK22/5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5952-81

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15217627

  • £112.32 / 50Āµg


EU Stock 5
Add to basket

Description

Description

Description: The IK22/5 monoclonal antibody reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.Applications Reported: This IK22/5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IK22/5 antibody has been tested by flow cytometric analysis on mouse CD254/TRANCE transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD254 (RANK Ligand)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
O35235
RP11-86N24.2, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, TNFSF11, TRANCE
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾Functional Assay
IK22/5
Unconjugated
Tnfsf11
Purified
Tnfsf11
IgG2a, kappa
50μg
Store at 2-8°C.
Primary
21943
Videos

For Research Use Only.