CD253 (TRAIL) Monoclonal Antibody (RIK-2), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9927-025

Product Code. 17111632

Description

The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not expressed by resting human cells but has been shown to be induced under certain activation conditions. Iin addition it is expressed on several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis. Applications Reported: The RIK-2 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of TRAIL in functional assays. Applications Tested: The RIK-2 antibody has been tested for blocking of staining with fluorochrome conjugated RIK-2 in flow cytometric analysis of human TRAIL transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD253 (TRAIL) Monoclonal antibody specifically detects CD253 (TRAIL) in Human samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: CD253 (TRAIL)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P50591
• Host Species: Mouse
• Quantity: 25 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: RIK-2
• Conjugate: Functional Grade
• Gene: TNFSF10
• Gene Alias: A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; TNFSF10; TNLG6A; Trail; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8743
• Content And Storage: 4° C
• Form: Liquid