Invitrogen™ CD25 Monoclonal Antibody (CD25-4E3), Brilliant Violet™ 605, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
30019247	Invitrogen™ 406-0257-42	100 Tests
30019901	Invitrogen™ 406-0257-41	25 Tests

Description

CD25 Monoclonal antibody specifically detects CD25 in Human, Non-human Primate samples. It is validated for Flow Cytometry

Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes. This monoclonal antibody is cross-reactive to rhesus monkey. Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CD25-4E3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Violet™ 605 (BV605) is a tandem dye that emits at 605 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (<a href='https://www.thermofisher.com/order/catalog/product/00-8222-49'>Product # 00-8222-49</a>) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href='https://www.thermofisher.com/order/catalog/product/00-5333-54'>Product # 00-5333-54</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically. Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation. Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser. BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Specifications

• Antigen: CD25
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: CD25-4E3
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 605
• Host Species: Mouse
• Target Species: Human, Monkey
• Gene Accession No.: P01589
• Gene ID (Entrez): 3559
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: IL2RA