BB700 Mouse Anti-Human CD25, Clone M-A251 (RUO)

Mouse monoclonal antibody that specifically binds to the 55 kDa type I transmembrane glycoprotein known as low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα).

Brand:  BD Biosciences 566447

Product Code. 15845059

Description

• The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα).
• CD25 is expressed on regulatory T cells, activated lymphocytes (T and B), and monocytes.
• It associates with the IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex.
• CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation.
• Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.
• The antibody was conjugated to BD Horizon BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.
• It is a polymer-based tandem dye developed exclusively by BD Biosciences.
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg., 695/40-nm filter).
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.

Specifications

• Antigen: CD25 (IL-2 Receptor α)
• Classification: Monoclonal
• Conjugate: Brilliant Blue 700 (BB700)
• Quantity: 100 Tests
• Primary or Secondary: Secondary
• Target Species: Human, Baboon, Cynomolgus, Rhesus Monkey
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: M-A251
• Host Species: Mouse
• Regulatory Status: RUO
• Test Specificity: CD25 (IL-2 Receptor α)
• Content And Storage: Refrigerate (2 to 8°C)