Invitrogen™ CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
17151312	Invitrogen™ 16-5838-38	5 mg
17131312	Affymetrix eBioscience 16-5838-025	25 mg
17141312	Affymetrix eBioscience 16-5838-050	50 mg

Description

The eBioC1.7 MAb reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 MAb and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.

CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.

Specifications

• Antigen: CD244
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 51744
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: CD244

• Clone: eBioC1.7 (C1.7)
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: Q9BZW8
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody