Invitrogen™ CD243 (ABCB1) Monoclonal Antibody (UIC2), APC, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 17-2439-42

Product Code. 15528066

Description

Description: This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes. This antibody has been reported to inhibit MDR1-mediated efflux. Applications Reported: This UIC2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UIC2 antibody has been pre-titrated and tested This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on the multidrug resistant cell line 8226 DOX40 and its parent line 8226/S. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Specifications

• Antigen: CD243 (ABCB1)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08183
• Gene Symbols: ABCB1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5243
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: UIC2
• Conjugate: APC
• Gene: ABCB1
• Gene Alias: ABC superfamily; P-glycoprotein; ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; ATP binding cassette subfamily B member 1; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette transporter subfamily B, member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; ATP-dependent translocase ABCB1; multidrug resistance protein 1; bovine p-glycoprotein; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; Evi32; GP170; mdr; Mdr1; MDR1A; Mdr1b; Mdr3; mdr-3; multi-drug resistance 3; multidrug resistance p-glycoprotein; multidrug resistance protein 1; Multidrug resistance protein 1A; multidrug resistance protein 1B; Multidrug resistance protein 2; multidrug resistance protein 3; multidrug resistance protein MDR1; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Pgp; p-gp; PGP1; PGP2; PGY1; Pgy-1; Pgy1-1; PGY2; Pgy3; Pgy-3; Phospholipid transporter ABCB1
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ