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CD23, PerCP-eFluor™ 710, clone: B3B4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0232-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15385119

  • £140.60 / 100µg


Estimated Shipment
06-07-2018
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Description

Description

Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD23
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P20693
CD23, CD23A, CLEC4J, FCE2, IGEBF, BLAST-2, C-type lectin domain family 4, member J, CD23 antigen, fc-epsilon-RII, immunoglobulin E-binding factor, low affinity immunoglobulin epsilon Fc receptor, lymphocyte IgE receptor
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
B3B4
PerCP-eFluor 710
Fcer2a
Conjugated
Fcer2a
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14128
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