CD23 Rat anti-Mouse, PE, Clone: B3B4, BD

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15881319	BD Biosciences 561773	25 μg
15847028	BD Biosciences 553139	0.2 mg

Description

The B3B4 antibody reacts with CD23, the low affinity IgE Fc receptor (FcεRII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes. It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines. The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines. IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE. CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23. The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice. This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice. Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments. B3B4 mAb does not cross-react with rat or human IgE Fc Receptor.

Specifications

• Antigen: CD23
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Mouse
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: FcεRII; Fc-epsilon-RII; Fcer2a; Ly-42; Low-affinity IgE receptor; Fcer2

• Clone: B3B4
• Applications: Flow Cytometry
• Conjugate: PE
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.