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CD223 (LAG-3) Monoclonal Antibody (3DS223H), APC-eFluor™ 780, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£302.00
Specifications
Clone | 3DS223H |
---|---|
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Conjugate | APC-eFluor 780 |
Description
Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells). This 3DS223H antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).Specifications
3DS223H | |
Flow Cytometry | |
APC-eFluor 780 | |
Mouse | |
Human | |
3902 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P18627 | |
LAG3 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
LAG3 |
For Research Use Only.