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CD215 (IL-15Ra), PerCP-eFluor™ 710, clone: DNT15Ra, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-7149-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341390

  • £206.01 / 100µg


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Description

Description

Description: This DNT15Ra antibody reacts with the alpha subunit of the Mouse IL-15 Receptor. The IL-15 Receptor alpha subunit is unique to IL-15, while the other two subunits of the receptor, beta and gamma, are shared with IL-2. IL-15R signaling is mediated by Jak/STAT pathways, and results in the activation of neutrophils, dendritic cells, and macrophages. IL-15 has also been found to play a critical role in the development and survival of NK cells and CD8+ T cells.Although high levels of mouse IL-15 mRNA have been observed in many cell types, free IL-15 is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and only secreted in complex with IL-15 Receptor alpha. This chaperoning is necessary from the ER to the Golgi, through secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta-gamma subunits of the receptor, or can be secreted in a soluble form lacking the transmembrane domain of IL-15 Receptor alpha. IL-15 and IL-15 Receptor alpha are expressed by dendritic cells and macrophages in response to inflammatory stimuli.Our testing suggests that there may be strain differences in expression and staining of the Mouse IL-15 Receptor alpha. This DNT15Ra antibody has been tested on cells from C57Bl/6 mice. Staining has also been observed with this antibody after pre-incubation of NK cells with exogenous Mouse IL-15/IL-15R Complex, suggesting that DNT15Ra will recognize both Mouse IL-15 Receptor alpha and the Mouse IL-15/IL-15R Complex.Applications Reported: This DNT15Ra antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This DNT15Ra antibody has been tested flow cytometric analysis of mouse bone marrow derived dendritic cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD215 (IL-15Ra)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60819
IL-15RA, Interleukin-15 receptor subunit alpha, IL15RA, CD215
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Flow Cytometry
DNT15Ra
PerCP-eFluor 710
Il15ra
Liquid
Il15ra
IgG1, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16169
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