CD205 Monoclonal Antibody (205yekta), NovaFluor™ Red 755, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
17842964	Invitrogen M051T02R06	25 μg
17872944	Invitrogen M051T03R06	100 μg

Description

The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells. Applications Tested: This 205yekta antibody has been tested by flow cytometric analyis of mouse bone marrow cells. This can be used at less than or equal to 0.2 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Use 5uL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. Excitation: 636 nm; Emission: 756 nm; Laser: 633-640 nm (Red) Laser

CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.

Specifications

• Antigen: CD205
• Concentration: 0.1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CA-IX; CD205; CLEC13B; C-type lectin domain family 13 member B; DCL-1 fusion; DEC205; DEC-205; DEC-205/CD205 protein; gp200; gp200-MR6; group VI C-type animal lectin; LY75; ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RGD1565962; RP23-10L17.4
• Gene Symbols: LY75
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: 205yekta
• Applications: Flow Cytometry
• Conjugate: NovaFluor Red 755
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q60767
• Gene ID (Entrez): 17076
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Gene: LY75