CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15277297	Affymetrix eBioscience 14-2029-82	100 μg
15267297	Affymetrix eBioscience 14-2029-80	25 μg

Description

Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells. Applications Reported: This 33.1 antibody has been reported for use in western blotting. Applications Tested: This 33.1 antibody has been tested by immunoblotting of reduced cell lysate prepared from the bEND3 cell line at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Specifications

• Antigen: CD202b (TIE2)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q02763, Q02858
• Gene Symbols: TEK
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: TEK

• Clone: 33.1
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Porcine, Rabbit, Rat
• Gene ID (Entrez): 100356675, 21687, 396729, 7010, 89804
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.