CD20, clone: 2H7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0209-82

Product Code. 15276847

Description

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: The 2H7 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.Applications Tested: The 2H7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD20
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P11836
• Gene Alias: B1, Bp35, CD20, LEU-16, MGC3969, MS4A1
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Immunohistochemistry (Frozen)
• Clone: 2H7
• Conjugate: Unconjugated
• Gene: MS4A1
• Format: Purified
• Gene Symbols: MS4A1
• Isotype: IgG2b, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 931

For Research Use Only.