CD195 (CCR5), PerCP-eFluor™ 710, clone: HM-CCR5 (7A4), eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 46-1951-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells. The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5.Applications Reported: This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolated- induced peritoneal exudate cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|C-C CKR-5, CC-CKR-5, HIV-1 fusion coreceptor, CHEMR13, CD195 antigen, CMKBR5|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.