CD184 (CXCR4) Monoclonal Antibody (12G5), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15588296	Affymetrix eBioscience 17-9999-41	25 Tests
15598296	Affymetrix eBioscience 17-9999-42	100 Tests

Description

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates. Applications Reported: This 12G5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of human lysed whole blood. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD184 (CXCR4) Monoclonal antibody specifically detects CD184 (CXCR4) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD184 (CXCR4)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: b2b220Clo; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor (LCR1); chemokine receptor 4; chemokine receptor CXCR4; Cmkar4; CXC; CXC chemokine receptor; C-X-C chemokine receptor type 4; CXC motif chemokine; C-X-C motif chemokine receptor 4; CXCR4; CXC-R4; CXCR-4; D2S201E; FB22; Fusin; HM89; HSY3RR; HUMSTR; LAP3; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; pre-B-cell-derived chemokine receptor; SDF-1 receptor; Sdf1r; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; WHIM; WHIMS
• Gene Symbols: Cxcr4
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Cxcr4

• Clone: 12G5
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P61073
• Gene ID (Entrez): 7852
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.