CD180 (RP105), clone: MHR73-11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1809-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MHR73-11 monoclonal antibody reacts with human CD180 (RP105). This 105kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on B, monocytes/macrophages, and dendritic cells. Histological studies show that RP105 is expressed mainly on mature B cells in mantle zones, while germinal center cells are either dull or negative. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. MHR73-11 activates B cells, leading to increases in cell size, expression of the costimulatory molecule CD80, and DNA synthesis. Moreover, ligation of RP105 protects B cells from irradiation- or dexamethasone-induced apoptosis. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death. A significant proportion of circulating B cells in SLE patients is RP105 negative. Loss of RP105 is associated with B cell activation and increased disease activity in SLE patients.Applications Reported: MHR73-11 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. MHR73-11 has also been reported in in vitro functional studies. (Please use Functional Grade purified MHR73-11, cat. 16-1809, in functional assays.).Applications Tested: The MHR73-11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|LY64, Ly78, MGC126233, MGC126234, RP105|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.