CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5911-050

Product Code. 17191352

Description

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported. Applications Reported: MFL3 has been reported for use in flow cytometric analysis. It has also been reported in blocking of the FasL mediated killing in functional assays. Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD178 (Fas Ligand) Monoclonal antibody specifically detects CD178 (Fas Ligand) in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: CD178 (Fas Ligand)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P41047
• Host Species: Armenian Hamster
• Quantity: 50 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: MFL3
• Conjugate: Functional Grade
• Gene: Fasl
• Gene Alias: ADAM10-processed FasL form; ALPS1B; APL; apoptosis (APO-1) antigen ligand 1; Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand); apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 ligand; CD95L; CD95-L; CD95L protein; fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fasl; Fas-L; FasL ICD; FasL intracellular domain; Faslg; Fas-LG; generalized lymphoproliferative disease; gld; mutant tumor necrosis factor family member 6; Receptor-binding FasL ectodomain; sFasL; Soluble Fas ligand; SPA; SPPL2A-processed FasL form; TNFL; Tnfsf6; TNLG1A; Tumor necrosis factor (ligand) superfamily member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor ligand; tumor necrosis factor ligand 1A; tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14103
• Content And Storage: 4° C
• Form: Liquid