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CD170 (Siglec F), clone: 1RNM44N, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1702-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15527436

  • £70.08 / 25Āµg


EU Stock 2
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Description

Description

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This 1RNM44N antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 μg/mL. This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells and can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD170 (Siglec F)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q920G3
OB-BP2, CD33L2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾Immunohistochemistry (Frozen)Ć¾Immunohistochemistry (Frozen)
1RNM44N
Unconjugated
Siglecf
Purified
Siglecf
IgG2a, kappa
25μg
Store at 2-8°C.
Primary
233186
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