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CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-1601-81
Code : Z2
Additional Details : Weight : 0.01000kg
Description
Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus. Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, functional assays, immunoprecipitation, and immunoblotting (WB). Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.
Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.Specifications
CD160 | |
Monoclonal | |
1 mg/mL | |
PBS with no preservative; pH 7.2 | |
O88875 | |
Cd160 | |
Affinity chromatography | |
RUO | |
54215 | |
4° C | |
Liquid |
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot | |
eBioCNX46-3 (CNX46-3) | |
Functional Grade | |
Cd160 | |
NK1, NK28, CD160 antigen, CD160 transmembrane isoform, CD160-delta Ig, natural killer cell receptor BY55, natural killer cell receptor | |
Rat | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.