CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 16-1601-81

Product Code. 15278577

Description

Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus. Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, functional assays, immunoprecipitation, and immunoblotting (WB). Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.

Specifications

• Antigen: CD160
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: O88875
• Gene Symbols: Cd160
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54215
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: eBioCNX46-3 (CNX46-3)
• Conjugate: Functional Grade
• Gene: Cd160
• Gene Alias: NK1, NK28, CD160 antigen, CD160 transmembrane isoform, CD160-delta Ig, natural killer cell receptor BY55, natural killer cell receptor
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.